Journal: PLoS ONE

Article Title: Identification of leaky gut-related markers as indicators of metabolic health in Dutch adults: The Nutrition Questionnaires plus (NQplus) study

doi: 10.1371/journal.pone.0252936

Figure Lengend Snippet: LGM levels (mean ± SE) in individuals with healthy (n = 40) and unhealthy (n = 40) metabolic profiles in the pilot study.

Article Snippet: Enzyme-linked immunosorbent assay was used to measure zonulin (K5601; Immundiagnostik AG, Bensheim, Germany) in serum as well as LBP (HK315-02; Hycult Biotech, Uden, Netherlands), EndoCAb IgM (HK504-IgM; Hycult Biotech), EndoCAb IgG (HK504-IgG; Hycult Biotech), EndoCAb IgA (HK504-IgA; Hycult Biotech), BPI (HK314-02; Hycult Biotech), sCD14 (HK320-02; Hycult Biotech), and PG (ABIN1116409; Antibodies-Online, Atlanta, GA, USA) in EDTA plasma according to the manufacturer’s protocol.

Techniques:

Journal: Scientific Reports

Article Title: Metagenomic analysis and identification of emerging pathogens in blood from healthy donors

doi: 10.1038/s41598-020-72808-8

Figure Lengend Snippet: Schematic workflow representation. Schematic representation of the workflow for blood sample preparation, genomic extraction and metagenomics analysis using Illumina HiSeq 4000 high-throughput sequencing. CMV antibodies and CMV DNA were analyzed via ELISA and quantitative real-time PCR, respectively.

Article Snippet: The CMV antibodies in specimen serum were detected using an anti-CMV IgG/IgM ELISA kit following the manufacturer’s protocol (Human anti-cytomegalovirus antibody IgG ELISA Kit,) and Human anti-cytomegalovirus antibody IgM ELISA Kit, Cusabio, USA).

Techniques: Sample Prep, Extraction, Next-Generation Sequencing, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction

Journal: Scientific Reports

Article Title: Metagenomic analysis and identification of emerging pathogens in blood from healthy donors

doi: 10.1038/s41598-020-72808-8

Figure Lengend Snippet: Clinical characteristics of CMV-IgG+, CMV-IgM+ and CMV-NAT+ in blood from healthy donors. The IgG and IgM antibodies of CMV in the blood samples were measured by ELISA. CMV DNA was detected by quantitative real-time PCR. No significant differences in the positivity rates were detected among sex, age, residence, profession and ethnicity. Of the 2986 positive samples (IgG or IgM), the positive rate of CMV-IgG, CMV-IgM and both CMV-IgG and CMV-IgM was 46.25%, 5.82%, 0.07%, respectively. Decreased CMV DNA was noted in the positive specimens (7.56 × 10 2 to 3.58 × 10 3 copies/mL). The positive rate of CMV-IgG was 78.91% in age 45–55 group, it’s higher than other age groups.

Article Snippet: The CMV antibodies in specimen serum were detected using an anti-CMV IgG/IgM ELISA kit following the manufacturer’s protocol (Human anti-cytomegalovirus antibody IgG ELISA Kit,) and Human anti-cytomegalovirus antibody IgM ELISA Kit, Cusabio, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction

Journal: The American Journal of Tropical Medicine and Hygiene

Article Title: Coinfection of Malaria and Bacterial Pathogens among Acute Febrile Patients in Selected Clinics in Ghana

doi: 10.4269/ajtmh.23-0099

Figure Lengend Snippet: Coxiella burnetii and Leptospira spp. detection in paired sera

Article Snippet: An ELISA was performed using commercially available kits , as follows: The Human Leptospira Antibody (IgM and IgG) ELISA kit (Cusabio, Wuhan, China) was used for the qualitative determination of human Leptospira antibody concentrations in serum.

Techniques:

Journal: The Journal of International Medical Research

Article Title: Incidence and characteristics of hepatitis E virus infection in children in Assiut, Upper Egypt

doi: 10.1177/0300060516659575

Figure Lengend Snippet: Prevalence of different types of hepatitis among 123 children with acute viral hepatitis. HEV, hepatitis E virus; HAV, hepatitis A virus; HBV, hepatitis B virus; CMV, cytomegalovirus; AIH, autoimmune hepatitis.

Article Snippet: Cytomegalovirus (CMV) infection was diagnosed in the presence of CMV IgM Human Anti-cytomegalovirus IgM antibody (anti-CMV-IgM) ELISA Kit; MyBioSource, Inc. San Diego, USA.

Techniques: Virus

Journal: PLOS Neglected Tropical Diseases

Article Title: Serological diagnosis of soil-transmitted helminth ( Ascaris , Trichuris and hookworm) infections: A scoping review

doi: 10.1371/journal.pntd.0012049

Figure Lengend Snippet: Glossary of the key terms used in this scoping review.

Article Snippet: Human anti-ascaris lumbricoides antibody(IgM) Elisa Kit , AFG Bioscience , Commercial kit , Recombinant protein: ACJ03764 , IgM , ≥82.1% , ≥85.7%.

Techniques: Biomarker Discovery, Clinical Proteomics, Diagnostic Assay, Enzyme-linked Immunosorbent Assay

Journal: PLOS Neglected Tropical Diseases

Article Title: Serological diagnosis of soil-transmitted helminth ( Ascaris , Trichuris and hookworm) infections: A scoping review

doi: 10.1371/journal.pntd.0012049

Figure Lengend Snippet: The number of records by technology principle applied ( panel A ), target analyte detected ( panel B ) and analyte detection agent used ( panel C ). The target analyte refers to the specific analyte that a test is designed to detect or measure, while the analyte detection agent refers to the product used in the test to investigate the presence of the target analyte. The color indicates the STH species targeted in each record. In cases where a record applied multiple options, the records was included in the count of all relevant options. FAT: fluorescent antibody test, ELISA: enzyme-linked immunosorbent assay, MFI: multiplex flow immunoassay, qSAT: quantitative suspension array technology, SDS-PAGE: sodium dodecyl sulfate polyacrylamide gel electrophoresis, REIA: reverse enzyme immunoassay, RIA: radioimmunoassay, Ab: antibody, Ig: immunoglobulin, IC: immunecomplex, ES: excretory/secretory, HW: hookworm, STH: soil-transmitted helminth. provides an overview of the 85 records used to create the figures.

Article Snippet: Human anti-ascaris lumbricoides antibody(IgM) Elisa Kit , AFG Bioscience , Commercial kit , Recombinant protein: ACJ03764 , IgM , ≥82.1% , ≥85.7%.

Techniques: Enzyme-linked Immunosorbent Assay, Multiplex Assay, Suspension, SDS Page, Polyacrylamide Gel Electrophoresis, RIA Assay

Journal: PLOS Neglected Tropical Diseases

Article Title: Serological diagnosis of soil-transmitted helminth ( Ascaris , Trichuris and hookworm) infections: A scoping review

doi: 10.1371/journal.pntd.0012049

Figure Lengend Snippet: Summary of all the in-house assays for which diagnostic sensitivity and specificity were assessed. Records are clustered based on target species, technology principle and analyte detection agent. HW: hookworm, LMS: larva migrans syndrome, FAT: fluorescent antibody test, ELISA: enzyme-linked immunosorbent assay, REIA: reverse enzyme immunoassay, qSAT: quantitative suspension array technology, Ig: immunoglobulin, ES: excretory/secretory, SD: standard deviation, STH: soil-transmitted helminth, ROC: Receiver Operating Characteristic, Ref: reference.

Article Snippet: Human anti-ascaris lumbricoides antibody(IgM) Elisa Kit , AFG Bioscience , Commercial kit , Recombinant protein: ACJ03764 , IgM , ≥82.1% , ≥85.7%.

Techniques: Diagnostic Assay, Enzyme-linked Immunosorbent Assay, Suspension, Standard Deviation, Infection, Sandwich ELISA, Western Blot, Recombinant

Journal: PLOS Neglected Tropical Diseases

Article Title: Serological diagnosis of soil-transmitted helminth ( Ascaris , Trichuris and hookworm) infections: A scoping review

doi: 10.1371/journal.pntd.0012049

Figure Lengend Snippet: Overview of the commercially available ELISAs for soil-transmitted helminthiases. Records were retrieved by a Google keyword search. A limitation of our search strategy is that records that were buried deeper in the search rankings are potentially omitted. However, we deem our approach comprehensive and inclusive for our study’s specific objectives. NA: information not available. ELISA: enzyme-linked immunosorbent assay.

Article Snippet: Human anti-ascaris lumbricoides antibody(IgM) Elisa Kit , AFG Bioscience , Commercial kit , Recombinant protein: ACJ03764 , IgM , ≥82.1% , ≥85.7%.

Techniques: Enzyme-linked Immunosorbent Assay, Biomarker Discovery, Diagnostic Assay, Recombinant